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reactive oxygen species ros detection kit  (Wanleibio)

 
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    Wanleibio reactive oxygen species ros detection kit
    Reactive Oxygen Species Ros Detection Kit, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reactive+oxygen+species+ros+detection+kit/cell+cycle+detection+kit/pm42097773-79-1-10
    Average 86 stars, based on 1 article reviews
    reactive oxygen species ros detection kit - by Bioz Stars, 2026-08
    86/100 stars

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    The results of the cellular validation experiments. (A,G) LCN2 expression levels in BEAS-2B cells after co-culture with nano-Fe 2 O 3 for 24 hours. (B,H,I) Expression profiles of GPX4 and FTH1 in BEAS-2B cells following co-culture with nano-Fe 2 O 3 for 24 hours. (E,K) Immunofluorescence validation of GPX4 expression in BEAS-2B cells after co-culture with nano-Fe 2 O 3 for 24 hours. (F,L) Immunofluorescence validation of FTH1 expression in BEAS-2B cells after co-culture with nano-Fe 2 O 3 for 24 hours. (C) Changes in ROS levels in BEAS-2B cells following co-culture with nano-Fe 2 O 3 for 24 hours. (D) Mitochondrial alterations in BEAS-2B cells observed via electron microscopy after co-culture with nano-Fe 2 O 3 for 24 hours. In the treated group, mitochondria (red arrows) exhibited discernible alterations such as a reduction in cristae and swelling (green arrows), compared to those in untreated cells. (J) Alterations in intracellular Fe 3+ levels in BEAS-2B cells following co-culture with nano- Fe 2 O 3 for 24 hours. *, P<0.05; **, P<0.01. BEAS-2B, bronchial epithelial cells, adenovirus 12-SV40 2B immortalized; DAPI, 4’,6-diamidino-2-phenylindole; ROS, reactive oxygen species.

    Journal: Translational Cancer Research

    Article Title: Molecular mechanisms of inhalable iron-containing particles driving lung carcinogenesis through the LCN2 signaling axis revealed by transcriptomic analysis

    doi: 10.21037/tcr-2025-2152

    Figure Lengend Snippet: The results of the cellular validation experiments. (A,G) LCN2 expression levels in BEAS-2B cells after co-culture with nano-Fe 2 O 3 for 24 hours. (B,H,I) Expression profiles of GPX4 and FTH1 in BEAS-2B cells following co-culture with nano-Fe 2 O 3 for 24 hours. (E,K) Immunofluorescence validation of GPX4 expression in BEAS-2B cells after co-culture with nano-Fe 2 O 3 for 24 hours. (F,L) Immunofluorescence validation of FTH1 expression in BEAS-2B cells after co-culture with nano-Fe 2 O 3 for 24 hours. (C) Changes in ROS levels in BEAS-2B cells following co-culture with nano-Fe 2 O 3 for 24 hours. (D) Mitochondrial alterations in BEAS-2B cells observed via electron microscopy after co-culture with nano-Fe 2 O 3 for 24 hours. In the treated group, mitochondria (red arrows) exhibited discernible alterations such as a reduction in cristae and swelling (green arrows), compared to those in untreated cells. (J) Alterations in intracellular Fe 3+ levels in BEAS-2B cells following co-culture with nano- Fe 2 O 3 for 24 hours. *, P<0.05; **, P<0.01. BEAS-2B, bronchial epithelial cells, adenovirus 12-SV40 2B immortalized; DAPI, 4’,6-diamidino-2-phenylindole; ROS, reactive oxygen species.

    Article Snippet: Total cellular ROS levels were measured using the ROS detection kit containing 2′,7′-dichlorofluorescin (DCFH-DA) (E-BC-K138-F, Elabscience, Houston, USA).

    Techniques: Biomarker Discovery, Expressing, Co-Culture Assay, Immunofluorescence, Electron Microscopy